FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_chip_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40658740
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3993020.9820815893458578TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG466550.064.24646
CGTATGC473950.063.6480444
GTATGCC479200.063.3452145
ATGCCGT466950.063.17213447
TGCCGTC462500.062.9924548
TAGGCAT567500.058.65651335
TCTCGTA511750.058.1567541
GCCGTCT493700.057.83466749
CATCTCG526200.057.51019739
CACTTAG583000.057.39105231
GCATCTC547700.055.74435838
CTCGTAT538050.055.46975342
ATCTCGT545400.055.13902340
AGTCACT613800.054.5799728
TCGTATG545300.054.24932543
CCGTCTT544400.053.4125550
TTAGGCA627450.053.40336234
TCACTTA642150.052.24671630
CTCCAGT643250.052.15173324
ACTCCAG645150.052.05188423
Produced by FastQC (version 0.11.2)