FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_chip_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442873
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2669531.1894778355694478TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG300550.064.759446
CGTATGC302450.064.4328644
TGCCGTC302300.064.24557548
ATGCCGT304050.064.0258747
GTATGCC304750.063.9810345
TCTCGTA314850.060.28648441
ATCTCGT324150.058.56711240
AGTCACT340000.057.4799928
AATCTCG331400.057.25416639
GCCGTCT340950.057.034749
ACTGACC346750.056.36143532
CTCGTAT347000.055.05267742
TCGTATG359050.054.64668343
CTGACCA358300.054.55411533
ACGTCTG360850.054.53837615
CCGTCTT361200.053.92352350
CGTCTGA365900.053.8045516
CTGAACT368700.053.4523119
ACTCCAG371000.052.94039523
TGCTTGA366550.052.78354358
Produced by FastQC (version 0.11.2)