FastQCFastQC Report
Mon 2 May 2016
H7237BGXY_n01_tga1_rep3_chip_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23852947
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1665650.698299459601365TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG210100.062.45430846
CGTATGC214850.061.38177544
GTATGCC220700.060.8333845
ATGCCGT212700.060.04547547
TGCCGTC210650.059.6321548
ACCGATG278850.056.85666732
GCCGTCT221050.055.33855449
CGATGTA288500.054.8939934
GTCACCG289150.054.8919829
TCTCGTA239850.054.3735841
CCGATGT294000.053.92692633
AGTCACC296050.053.6122828
CTCGTAT250850.051.9473942
ATCTCGT262200.050.73909440
TCACCGA315800.050.44800630
TCGTATG252750.050.07376543
TATCTCG270600.049.8368539
CACCGAT324750.049.00399431
CCGTCTT258900.048.97841650
CAGTCAC328050.048.34969327
Produced by FastQC (version 0.11.2)