FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_chip_180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39243081
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3392290.8644300889626887TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC454230.11574779258539869No Hit
CCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAA405250.10326661150789868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC376400.065.26565644
TATGCCG376300.065.13492646
GTATGCC379600.064.70624545
TGCCGTC377750.064.6623748
ATGCCGT378950.064.615247
CACGCCA395100.062.5570731
TCACGCC398650.061.92098230
TCTCGTA396800.061.81638341
GCCAATA408900.060.3862234
ATCTCGT407700.060.21467640
CCAATAT440900.056.09864435
TGCTTGA437150.055.82958
GCTTGAA440700.055.60115459
CTGAACT476450.052.3973119
ACGTCTG483100.051.61847715
CGTCTGA484500.051.49086416
TCTGAAC487250.051.26457218
AATATCT484050.051.0858237
GCCGTCT479650.050.88865749
AGTCACG500800.049.69550328
Produced by FastQC (version 0.11.2)