FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_chip_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33900542
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC3072810.9064191363076142TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG354750.062.9753146
CGTATGC359950.062.13296544
ATGCCGT361750.061.8343947
GTATGCC362950.061.60011745
TGCCGTC364900.061.32874748
GCCGTCT394450.056.72521649
TCTCGTA395500.055.6030641
CTCGTAT413200.053.65315242
ATCTCGT416350.052.86005440
TCGTATG426700.052.7510143
ATCACGA430800.051.80787734
CCGTCTT437250.051.31571650
TCACGAT436800.050.90347335
CACGATC437200.050.14577536
CATCACG446350.050.13628833
GTCACAT448000.050.0218729
CGATCTC457150.048.0655238
ACGATCT465950.047.18070237
ACTCCAG480150.046.897723
CTCCAGT480800.046.8200124
Produced by FastQC (version 0.11.2)