FastQCFastQC Report
Mon 7 Mar 2022
H722TBGXL_n01_AKM331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH722TBGXL_n01_AKM331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18640091
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT534190.28658121894361993TruSeq Adapter, Index 27 (97% over 44bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA413460.2218122218394749No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA359760.1930033496081108No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT215030.11535887888100976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)