FastQCFastQC Report
Mon 7 Mar 2022
H722TBGXL_n01_AKM330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH722TBGXL_n01_AKM330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18955057
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA451040.23795233113780667No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA352360.1858923452459151No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT320790.16923715924462795TruSeq Adapter, Index 25 (97% over 44bp)
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT277800.1465571957921308No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG190750.10063277572839796No Hit

[OK]Adapter Content

Adapter graph