FastQCFastQC Report
Mon 7 Mar 2022
H722TBGXL_n01_AKM329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH722TBGXL_n01_AKM329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17778981
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT596440.33547479464655483TruSeq Adapter, Index 7 (97% over 36bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA447730.25183108075766547No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA246960.1389055986954483No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT213310.11997875468790926No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG188100.10579908938538153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)