FastQCFastQC Report
Mon 7 Mar 2022
H722TBGXL_n01_AKM325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH722TBGXL_n01_AKM325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17310314
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1353610.7819673288422152TruSeq Adapter, Index 16 (97% over 40bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA491880.2841542909042551No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA417330.24108748113985684No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT306380.1769927454811045No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG239270.1382239513390687No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG199650.11533586276944484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)