FastQCFastQC Report
Mon 7 Mar 2022
H722TBGXL_n01_AKM322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH722TBGXL_n01_AKM322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17654443
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA453420.2568305326880038No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA336980.1908754640404118No Hit
TTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTCCTT254800.14432627526113398No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT232560.13172887980662998TruSeq Adapter, Index 13 (97% over 40bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG206090.11673548692530258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)