FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59686106
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1271780.21307806543787597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG58600.019.821312
CCCGAGC62550.019.018151
CCCGGAC78900.014.9962321
GCCCGGA83450.014.4717620
TGCCCGG83400.014.43802719
CGGACAG103450.011.94525323
CCGGACA106700.011.41733822
CGAGCGT108250.011.1501283
CACTCCG117300.09.93715518
GCGCACT115250.09.86985915
GAGCGGC127950.09.38339964
TTGCCCG133550.09.30465518
CGCACTC127800.09.17516
TCCGCAC133850.08.96865152
CCAGCGC133350.08.765964512
GAGCGTG144750.08.6044554
CGCACCA147450.08.28336654
AGCGCAC138950.08.23668814
CAGCGCA148250.08.19203213
GCGTGAA150400.08.1882986
Produced by FastQC (version 0.11.2)