FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54535205
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG969650.17780257725262058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG65300.021.1081372
CCCGAGC70950.019.6251541
CCCGGAC82200.016.8216421
TGCCCGG88250.015.9054819
GCCCGGA89850.015.54484320
CGGACAG107750.013.45060123
CCGGACA110550.013.01483122
CGAGCGT114150.012.2590773
GCGCACT120400.012.20928315
CACTCCG126750.011.76466618
TCCCGAG75500.011.723385
GAGCGGC130000.011.20120464
CCAGCGC135900.011.15102112
CGCACTC133600.011.08214916
AGCTTTG318350.011.0444391
TTGCCCG131350.010.95291518
AGCTTTC297500.010.9129831
AGCTTAC161250.010.7829881
AGCTTCC292900.010.558821
TCCGCAC139800.010.36432552
Produced by FastQC (version 0.11.2)