FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63154496
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1826660.28923673145930895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG54300.017.0734672
CCCGAGC57550.016.8393611
CCCGGAC79400.012.2567221
GCCCGGA85100.011.229506520
TGCCCGG85750.011.06251319
CCGGACA108200.09.22095322
CGGACAG106150.09.10224823
CGAGCGT112500.08.5207443
GCGCACT110500.07.76021815
GAGCGGC136150.07.301471764
CACTCCG117850.07.27698318
GAGCGTG140950.07.24774274
TTGCCCG144700.07.01526918
CGCACTC123100.06.93775216
AGCGTGA156350.06.84711845
GCGTGAA153350.06.84444576
TCCGCAC143000.06.77944152
CCAGCGC138250.06.68322112
CGCACCA154700.06.605711554
AGCTTTG345650.06.54875761
Produced by FastQC (version 0.11.2)