FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72658473
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1177810.1621022230951647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC66600.016.3902871
CCGAGCG63150.016.2325042
CCCGGAC88500.012.42002921
GCCCGGA94250.011.43908320
TGCCCGG92600.011.41580219
CGGACAG114000.09.61148823
CCGGACA119700.09.27068422
CGAGCGT117500.09.1112723
TTGCCCG152650.07.612944618
GAGCGGC148100.07.39780364
TCCGCAC158100.06.928976552
CGCACCA169300.06.904326454
GAGCGTG160350.06.80742934
AGCGTGA174000.06.47435765
AGCTTTC370850.06.44360071
GCGTGAA174150.06.4288066
GCGCACT118900.06.328816415
CCGCACC176600.06.18298753
AGCTTTG400200.06.16336971
AGCTTAC191900.06.0712131
Produced by FastQC (version 0.11.2)