FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623658
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530610.42032982832709825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG131750.021.15029770
TAGGGGG73900.020.13197738
GGGGTTA79850.018.75905454
GGGGTAA80450.017.61858654
AAAGGGG135850.017.5991969
TTAGGGG39350.017.52542537
GGGTTAA65550.017.35164655
ATGGGGG94650.017.16134670
GGGGGTA129700.016.0012353
AATGGGG91200.015.35391570
TGTGGGG122550.014.79683136
TTTGGGG109050.014.34941336
TTTAGGG42300.013.98616436
ATAGGGG50700.013.73968469
AAAAGGG179850.013.50719468
GGGTAAA90700.013.35053955
GGTTAAA84150.013.22538156
TTGGGGG172700.012.77012937
GTAGGGG41500.012.23114237
GGGTTGT73600.012.17497130
Produced by FastQC (version 0.11.2)