FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68066431
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG142750.022.0815435
CTCCCGA116300.020.6058864
TCTCCCG129050.018.3257393
AGCTTTG431050.016.0945451
AGCTTAC200700.015.8452881
AGCTTGC317550.014.950351
AGCTTAT313850.014.5803931
AGCTTTA433900.014.4649331
AGCTTGT355700.014.4091181
AGCTTCC365250.014.2526731
AGCTTTC373250.013.8909521
AGCTTAG139500.013.8185031
AGCTTGG303450.013.8003671
AGCTTTT667700.013.5811721
AGCTTCG267700.013.21254351
CCCGAGC137700.012.8307276
AGCTTAA242600.012.4885141
ATCTCCC205800.011.9843012
AGCTTGA413550.011.8266781
CACGGAG103050.011.81900513
Produced by FastQC (version 0.11.2)