FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64631227
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG881620.13640774605749015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG139700.023.7665675
AGCTTAC214200.021.6591051
CTCCCGA114800.021.363474
TCTCCCG125450.019.60553
AGCTTTG428650.019.597731
AGCTTGC323650.019.0370751
AGCTTTA422950.018.5051841
AGCTTGT362050.018.3032361
AGCTTAT320100.017.8600581
AGCTTCC373500.017.3486981
AGCTTGG297300.017.2761751
AGCTTTC367300.017.0128461
AGCTTAG141950.016.859211
AGCTTTT641150.015.9672881
AGCTTGA396350.015.8100481
CACGGAG107650.015.24794913
CCCGAGC139850.015.0608166
AGCTTAA258500.014.9696291
AGCTTCG269650.014.8696481
TAGGTCC115550.014.60212834
Produced by FastQC (version 0.11.2)