FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49867692
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1808930.36274588364747257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG130100.025.9504815
TAGGGGG248550.024.22560738
CTCCCGA94000.022.7782484
AGCTTTG308800.021.3120521
AGCTTAC142750.020.9312651
AGCTTTT513800.020.7351381
AAGGGGG454050.020.46236870
AGCTTAT231100.020.4384331
AGCTTTA302350.020.1929231
TTAGGGG160250.019.48630137
TCTCCCG110650.019.3821663
AGCTTCC272550.018.8192161
AGCTTTC273300.018.4347231
AGCTTAG95300.018.0628361
GGGGTAA286800.017.58371254
GGGGTTA266700.017.3998854
AAAGGGG503800.016.87766369
AATGGGG322650.016.84994570
GGGTTAA234350.016.39680355
GGGGGTA442500.016.25257353
Produced by FastQC (version 0.11.2)