FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64713095
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG797420.12322390081945549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC143200.030.9319361
CCCGGAC152300.028.75394821
CCGAGCG160050.027.609022
GCCCGGA166000.026.40123420
TGCCCGG178300.024.38279519
CGGACAG186950.023.63130823
CGAGCGT201500.021.825233
CCGGACA204600.021.71234922
GAGCGTG210950.021.0797774
TTGCCCG207750.021.07798818
TCCGCAC225000.019.58351352
CGCACCA237950.018.7373754
GCGCACT168750.018.25163315
GCGTGAA250700.017.891576
AGAGCGG250150.017.51776763
CGCACTC174300.017.4906716
AGCTTTG349150.017.4462931
CCGCACC260800.017.27017853
GAGCGGC255050.017.20967564
GGACAGT261000.016.98007624
Produced by FastQC (version 0.11.2)