FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30806435
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120180.3636188348310994No Hit
GATCGTGGTTCGGTTTCGGCCGAGCCAAGGCGCCAAAAACACATAAGCAC631560.20500911579025616No Hit
GATCCATCGCTGAAGCTAGTACGTATTATCGAGATGCAAATCTCAACAAC532400.1728210356050611No Hit
GATCTTGTCCTAGTCCAGAAGCATCACAAGTCAAGACAGAGCTTCACCGT528540.1715680506361739No Hit
GATCTGCTTCTTACCAATCTTCCCGTTACCGCCACGCACTAAAGCTCCAA506430.16439097870298852No Hit
GATCAGCCGAGTCAAGTCGGACGCCATTTGGCAGCTCCTCCGATATAAGA490210.1591258449736232No Hit
GATCCTTTGGAGCTGAAGATTCAGTTCATAAGGGGGACCTTTGTGATCAG473630.1537438525424964No Hit
GATCACAAAGGTCCCCCTTATGAACTGAATCTTCAGCTCCAAAGGATCAG472540.15339003036216298No Hit
GATCTGCTTCTTACCAATCTTCCCGTTACCGCCACGCACTTAAGCTCCAA384600.12484404638186794No Hit
GATCCCGTCCCCCAATCAATATCATCACCTTCCCACATCTCCACATACGG366500.11896865054330369No Hit
GATCTTATTGTCAGATGGAACCTTCTTGGCATACCGTTCTCCACTGATGT362660.11772215772451439No Hit
GATCGGACGGGATGGCGTGCATTCAACGTGATATGGTCGTAAGCGTCTAT361230.11725796899251732No Hit
GATCGTAGACCTCGCTCGCTGTCTGCTGTAGGTTGGTGTTAGGCGCAGAG325960.10580906229493935No Hit
GATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTA315460.10240068349356231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCAC313750.065.24241
GATCCTA155650.064.733181
GATCCCT105350.064.158781
GATCGTT214200.064.139421
GATCATC419100.064.11021
GATCCAT320650.064.067591
GATCCTC222950.064.007341
GATCCTG200650.063.8328361
GATCTGC265550.063.6332931
GATCACT264850.063.56371
GATCTCT249950.063.5317151
GATCGTG179850.063.531531
GATCATT508650.063.491161
GATCTTC542900.063.481041
GATCTCC242900.063.431261
GATCTTG308000.063.245861
GATCTTA215850.063.227711
GATCTTT581150.063.1496241
GATCAAT463800.063.0758321
GATCCGT109850.062.8681451
Produced by FastQC (version 0.11.2)