FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19153845
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2903121.5156852318685883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGG172950.038.35788336
TTATAGG79150.031.97781835
TTAGGGG238050.031.38218137
TATAGGG113500.030.35045436
TTTTAGG178350.028.5202935
TATAAGG69650.028.39836336
TAGGGGG523600.028.0399138
AATGGGG453250.027.91231570
GGTTAAA332050.026.29725856
GTTTAGG69300.026.11672435
AAGGGGG842700.025.57653670
GGGTTAA348300.025.23073255
GGAGTAA123200.024.37380854
GTTAAAA442950.024.12205757
GGTAAAA455250.023.88551956
TTGTAGG85400.023.2017135
GGGTAAA440650.023.03248655
GGGATAG94150.022.90383323
GTAAAAA694500.022.65668357
TTTAAGG90750.022.64422436
Produced by FastQC (version 0.11.2)