FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18196072
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG854050.46935954089432047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGG20100.016.54572937
AAGGGGG87000.016.05510770
TAGGGGG40350.015.70317338
GGGGTTA49050.015.0548954
AAAGGGG81200.014.74387869
GGGTTAA42500.014.57477255
GGGGTAA49450.014.08383554
TTTAGGG16550.013.96072236
GGGGGTA70800.013.791182553
GTTAAAA49150.013.59937357
AAAAGGG90000.013.06819768
GGTTAAA44250.012.89125756
ATGGGGG70150.012.27628670
AAAAAGG121300.011.34104467
TTTGGGG64900.011.0578636
GGGTTTA28800.010.81471155
AATGGGG65550.010.573873569
AAAAAAG183550.010.20277566
GGGTAAA59700.010.19981655
TGTGGGG92350.09.855947536
Produced by FastQC (version 0.11.2)