FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n02_matt_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n02_matt_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24897376
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG546250.21940063081346403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA25250.019.3987461
GATCTAA33300.018.7017751
GATCTAT44950.016.5011121
GATCTAG28750.016.3069951
GATCTTT86750.014.6804351
GATCCAA67050.014.2974571
GATCCCA40200.014.1862821
GATCTTA30000.014.1114341
GATCCAT69700.013.6534731
GATCCTT61650.012.9959921
GATCTCT57300.012.944591
GATCTAC78750.012.928541
GATCAAT83850.011.7666571
GATCCAC72950.011.654351
GATCTCA61850.011.6529151
GATCTGT50650.011.5357031
GATCTGA70200.011.5128251
GATCCGA60950.011.0213431
GATCCGT52150.010.9355421
GATCAAA125800.010.8743471
Produced by FastQC (version 0.11.2)