FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59686106
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG62950.021.631322
CCCGAGC64650.021.5005261
CCCGGAC84700.016.1989721
GCCCGGA90650.015.174088520
TGCCCGG91600.015.13026819
CGGACAG111350.012.57388523
CGAGCGT116100.012.1499773
CCGGACA117900.011.84533922
GAGCGGC129650.010.63578564
TTGCCCG142450.010.36801118
TCCGCAC148600.09.65729852
GAGCGTG149400.09.6056994
GCGTGAA157900.09.3768166
CGCACCA154500.09.33371554
AGAGCGG154250.09.1891863
AGCGTGA161300.09.1791645
GCGCACT120700.08.95955715
CACTCCG126350.08.80840718
CGCACTC130850.08.77277916
CAGAGCG164450.08.74689262
Produced by FastQC (version 0.11.2)