FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54535205
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG64950.021.8814332
CCCGAGC68050.020.9918041
CCCGGAC83850.017.28161621
TGCCCGG87650.016.5308419
GCCCGGA89800.016.37012920
CGGACAG108700.013.46013423
CCGGACA110800.013.2680122
GCGCACT126700.013.12025615
CGAGCGT111700.012.91058353
CACTCCG135050.012.20573818
GAGCGGC123000.011.836843564
CGCACTC140850.011.653172516
CCAGCGC144400.011.34236412
CAGCGCA150850.011.13578613
TCCCGAG70800.011.0247975
AGCGCAC150400.011.00619114
TTGCCCG136600.010.99117918
AGCTTTC305800.010.5906341
TCCGCAC136650.010.55315452
GCGTGAA144300.010.5517086
Produced by FastQC (version 0.11.2)