FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63154496
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG58200.018.4645842
CCCGAGC60000.018.264671
CCCGGAC79450.013.70091921
GCCCGGA84650.012.9831520
TGCCCGG86250.012.53838719
CGGACAG109000.010.14750523
CCGGACA112100.010.0540922
CGAGCGT113500.09.8377693
TGGGGGG88200.08.4133395
GAGCGTG140900.08.3966114
GAGCGGC132400.08.37945964
TTGCCCG142950.07.785490518
TCCGCAC147350.07.62525352
GCGTGAA152350.07.58181146
AGCGTGA161300.07.42151985
GCGCACT121550.07.31321515
AGAGCGG156300.07.165327563
CGCACCA161450.07.08929854
AGGGGGG80100.07.0354925
CACTCCG126100.06.85527418
Produced by FastQC (version 0.11.2)