FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72658473
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG65450.017.1679462
CCCGAGC68350.016.8526521
CCCGGAC91150.012.55672521
GTCACGC115500.011.75797129
TGCCCGG97550.011.73193419
GCCCGGA97900.011.69079720
TCACGCC130000.010.47327730
CGGACAG117150.09.97932523
GCCGTCT140300.09.85443349
CGAGCGT120550.09.5817253
CCGGACA124200.09.55363922
TGCCGTC151600.09.2814848
AGTCACG159900.08.77775328
CACGCCA162550.08.52670631
TATCTCG176900.08.34904439
TGGGGGG101050.08.3131725
GTATGCC170450.08.27537545
GAGCGGC147800.07.790632264
GAGCGTG158850.07.7340994
TATGCCG192250.07.610169446
Produced by FastQC (version 0.11.2)