FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623658
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT4250723.3672648609460114TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC731580.5795309093449775TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA519050.063.5262135
CCGTACG610450.063.51627733
CGTACGT519300.063.4691734
CGTAATC501450.062.93407438
TACGTAA526100.062.65397636
ACCGTAC623750.062.31344232
GTAATCT505200.062.15443839
ACGTAAT528650.062.0609537
TAATCTC506600.061.85263440
AATCTCG506400.061.81559841
CACCGTA639650.060.9781431
AGTCACC655050.059.5901428
CAGTCAC661700.059.11822527
TCACCGT661800.058.9057430
GTCACCG667150.058.3667829
CCAGTCA673050.057.99126426
TCCAGTC681150.057.26523225
CTCCAGT689700.056.5498124
ACTCCAG698400.055.91486423
GTATGCC580300.055.90095547
Produced by FastQC (version 0.11.2)