FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68066431
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG143600.021.7907185
CTCCCGA118550.020.0768324
TCTCCCG131000.018.2493253
GTTTCGG269550.017.1913934
CGTTTCG285100.016.59756533
AGTCACG288600.016.59099828
GTCACGT295800.016.06860729
AGCTTGC315750.015.368581
AGCTTTG428900.014.922241
AGCTTAC197200.014.87822151
GTATGCC188850.014.7901947
CGGAATC308600.014.3579438
CTCGTAT206350.014.18016744
AGCTTGT353250.014.0146141
TATGCCG192700.013.98627248
TCGGAAT340000.013.88641837
TTTCGGA346600.013.72318235
AGCTTAT305500.013.4087571
AGCTTCC378950.013.38752651
AGCTTTA431100.013.3517341
Produced by FastQC (version 0.11.2)