FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64631227
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2089630.3233158485448528TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC376450.042.165347
GCCGTCT375200.041.4577651
TATGCCG393950.040.2392448
CGTATGC405000.039.11506746
CTCGTAT398200.038.8416144
TATCTCG395150.038.64407341
GGCCTTA412000.038.380336
TCTCGTA426600.036.15671543
TCGTATG442450.035.64578245
TGCCGTC446350.035.35143350
ATGCCGT456200.034.76410749
CCGTCTT452100.034.630652
CACGTGG476150.033.54843531
ACGTGGC474700.033.50282332
ATCTCGT466400.033.3563342
TCACGTG497050.032.46162430
GTGGCCT495000.032.0441534
CGTGGCC510200.031.21996733
AGTCACG520100.031.20544628
GCCTTAT511900.030.93787237
Produced by FastQC (version 0.11.2)