FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49867692
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT16927423.394466300946913TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2053100.065.3109247
CGTATGC2076600.064.6579746
CTCGTAT1990900.064.5566444
GCCGTCT2067500.064.525951
TATGCCG2079500.064.4219548
TCGTATG2086600.064.10268445
CCGTCTT2102900.063.43455552
TCTCGTA2010300.063.39258643
ATGCCGT2144100.062.5175749
TGCCGTC2160650.061.96013650
ATCTCGT2048100.061.7452142
CTGCTTG2179350.060.85839559
GTCTTCT2216500.060.05599254
CGTCTTC2225700.059.9874553
TGCTTGA2216500.059.96426860
GCTTGAA2230750.059.84346861
AACGATC2094600.059.7566338
ACGATCT2097050.059.44046439
TCTGCTT2236100.059.33956558
CGATCTC2110650.059.0228740