FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64713095
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTT713750.11029452385177992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC155750.033.6516151
CCCGGAC167250.031.05637421
CCGAGCG177550.029.4941922
GCCCGGA180450.028.7065120
TGCCCGG194450.026.79985619
CGGACAG199800.026.33056823
CCGGACA217000.024.19484722
GAGCGTG222200.023.486954
CGAGCGT225700.023.2317183
TTGCCCG227250.022.9008418
CGCACCA253500.020.4349154
GCGTGAA262700.020.3191326
AGAGCGG259350.019.99912563
GAGCGGC267350.019.36136664
GGACAGT282450.018.97282624
CCGCACC287250.018.3631153
GCGCACT176000.018.09505815
CAGAGCG287900.018.08883962
TCCGCAC288600.018.0832352
AGCGTGA298500.017.8704155
Produced by FastQC (version 0.11.2)