FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30806435
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1817730.5900487998692481No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1513870.49141356343244524TruSeq Adapter, Index 12 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1089560.3536793530312742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG635530.20629780758468158No Hit
GATCCATCGCTGAAGCTAGTACGTATTATCGAGATGCAAATCTCAACAAC628730.20409047655140883No Hit
GATCGTGGTTCGGTTTCGGCCGAGCCAAGGCGCCAAAAACACATAAGCAC589180.1912522497328886No Hit
GATCTTGTCCTAGTCCAGAAGCATCACAAGTCAAGACAGAGCTTCACCGT551030.17886847342121864No Hit
GATCAGCCGAGTCAAGTCGGACGCCATTTGGCAGCTCCTCCGATATAAGA520200.16886082404536584No Hit
GATCTGCTTCTTACCAATCTTCCCGTTACCGCCACGCACTAAAGCTCCAA513060.16654312646042946No Hit
GATCACAAAGGTCCCCCTTATGAACTGAATCTTCAGCTCCAAAGGATCAG500890.162592653125881No Hit
GATCCTTTGGAGCTGAAGATTCAGTTCATAAGGGGGACCTTTGTGATCAG489790.15898950982156812No Hit
GATCTGCTTCTTACCAATCTTCCCGTTACCGCCACGCACTTAAGCTCCAA408920.13273850090086697No Hit
GATCCCGTCCCCCAATCAATATCATCACCTTCCCACATCTCCACATACGG395710.12845043576122975No Hit
GATCGGACGGGATGGCGTGCATTCAACGTGATATGGTCGTAAGCGTCTAT389010.12627556547844632No Hit
GATCTTATTGTCAGATGGAACCTTCTTGGCATACCGTTCTCCACTGATGT383250.12440582625026234No Hit
GATCAGTATGCCGAGACGCGAATCTCAGCTCACTAATGTTGCCCGACAAG327860.10642581655423615No Hit
GATCGTAGACCTCGCTCGCTGTCTGCTGTAGGTTGGTGTTAGGCGCAGAG318120.10326413945657782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCAC313450.065.631631
GATCCTA156500.065.390421
GATCCAT323750.064.841271
GATCATC420500.064.675961
GATCCTC216800.064.530621
GATCATT516050.064.363071
GATCTGC265150.064.185011
GATCGTT197450.064.152131
GATCTTG297150.064.130151
GATCCCT107050.063.9708061
GATCCTG212350.063.9704061
GATCCCG144700.063.948191
GATCACT260650.063.909641
GATCTTC518550.063.7016831
GATCTCT236600.063.5991481
GATCAAT459850.063.511691
GATCTTT558850.063.4745451
GATCGTG166850.063.432461
GATCAGT241950.063.393871
GATCAAA731700.063.360731
Produced by FastQC (version 0.11.2)