FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19153845
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC417225621.78286396282313TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC886160.46265384313175756TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC334040.17439840408022514TruSeq Adapter, Index 9 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC321630.16791928722405344TruSeq Adapter, Index 9 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC311420.16258876481458423TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC240450.12553615214073205TruSeq Adapter, Index 9 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC217600.11360643254657225TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4577500.068.3457242
TCGTATG4748750.068.34492543
GTATGCC4753850.068.2549645
CGTATGC4765450.068.14908644
TCTCGTA4564850.068.1313441
TATGCCG4768100.068.0131346
GTCTTCT4747550.067.95376652
AGATCTC4562700.067.945338
CCGTCTT4757100.067.940250
TCTGCTT4736900.067.9296856
CAGATCT4559400.067.85914637
TGCTTGA4749850.067.8317558
GCCGTCT4767100.067.8147749
GCTTGAA4768350.067.7759759
TCTTCTG4762050.067.68255653
TTCTGCT4752800.067.6635155
CTGCTTG4761150.067.55434457
CTTGAAA4800450.067.5214460
ATCTCGT4590700.067.49364540
ATCAGAT4766650.067.4669135
Produced by FastQC (version 0.11.2)