FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18196072
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1834091.007959300226994TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTA292450.062.77084734
CTCGTAT236000.061.62003742
ACTAGCT298450.061.57927732
CTAGCTT298350.061.4946733
AGCTTAT297800.061.1846435
CTTATCT268800.060.610137
TCGTATG231100.060.4729443
CACTAGC305900.060.3332531
TCACTAG306900.060.1826130
TTATCTC268600.058.81814638
TCTCGTA252350.058.29286641
GTATGCC256750.057.800845
GTCACTA321650.057.79373629
GCTTATC312800.057.04216836
AAAAGGG210250.056.7274370
AGTCACT328600.056.5093328
CGTATGC262200.055.70453644
CTTGAAA258350.055.31217660
CAGTCAC352100.052.8773727
GTCTTCT248600.051.27862552
Produced by FastQC (version 0.11.2)