FastQCFastQC Report
Thu 28 Apr 2016
H5YVCBGXY_n01_matt_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YVCBGXY_n01_matt_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24897376
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCAA66150.017.1482891
GATCCTA22100.017.1094911
GATCTAA31150.015.51052951
GATCTTA27900.015.4349971
GATCCAT68850.015.3062291
GATCCCA42900.015.1796331
GATCTAT43100.015.0279621
GATCCCT28550.013.2441251
GATCTAC77300.013.0442351
GATCTTT84950.012.941121
GATCTAG27850.012.6970211
GATCCGT53450.012.5764821
GATCAAA121550.012.2128341
GATCTCT55900.011.6494861
AGGGGGG40950.011.6276151
GATCCTT58450.011.5006481
GATCCAC69300.011.3167151
GATCTGA72900.010.9019431
GATCAGA79600.010.8639951
GATCCGA62000.010.8421431
Produced by FastQC (version 0.11.2)