FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18936456
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC463340.2446814757735027TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53150.064.3365146
GTATGCC54100.063.8545545
CGTATGC53900.063.83241744
GCCGTCT52700.063.55760249
TGCCGTC53300.062.90846348
ATGCCGT54400.062.47265647
CCGTCTT58850.057.15248550
TCTCGTA57950.056.83308841
ATCTCGT58950.056.04755440
AATCTCG60900.054.310439
AGTCACC67550.053.6782528
TGTAATC65300.050.9728936
GCTTGAA69600.048.47514359
TGCTTGA69050.048.202658
CTTGTAA82550.044.01020434
ACGTCTG86000.042.73170515
CGTCTGA86300.042.58315716
TCTGAAC88500.041.5635918
CTTGAAA84250.040.54421660
ACACGTC91800.040.0313513
Produced by FastQC (version 0.11.2)