FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24385486
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC395380.1621374287967851TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47800.058.79592546
GTATGCC48100.058.79435745
TGCCGTC48100.058.7206348
ATGCCGT48450.058.15184447
GCCGTCT48600.057.82856849
CGTATGC49600.057.0876944
CACGGCT55550.050.90872631
GCTACAT57400.049.33033835
CATCTCG57600.048.97725339
TCTCGTA58400.048.3655741
CCGTCTT58750.048.31365650
CTACATC60700.046.706436
ATCTCGT61500.046.09855340
TACATCT64900.043.52200737
GCTTGAA72400.039.2035959
TGCTTGA72500.038.9083958
ACGTCTG84400.034.04526515
CGTCTGA85150.033.7866416
TCTGAAC88600.032.90622318
ACACGTC91300.031.39511113
Produced by FastQC (version 0.11.2)