FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22301926
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT22650.057.63792447
TATGCCG25650.056.76421446
GTATGCC26450.056.2387745
CGTATGC24950.055.13083644
TGCCGTC22650.055.01111648
GCCGTCT21900.054.1775549
GCTTATC51100.050.75383836
CACTAGC53400.049.87767831
GTCACTA56200.047.51711729
ACTAGCT56500.047.20371232
TAGCTTA56600.047.18278534
AGTCACT58300.046.0456628
CCGTCTT28950.044.9730550
AGCTTAT60200.044.47720735
ATCTCGT34000.043.8524140
TCTCGTA31850.043.84543241
CGTCTGA72350.037.24903516
ACGTCTG73600.036.5682815
TCTGAAC74550.036.14947518
TTATCTC45950.035.19031538
Produced by FastQC (version 0.11.2)