FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24098820
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC332130.13782002604276888TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC41150.060.558648
TATGCCG41350.060.26519446
CGTATGC41650.060.08532744
ATGCCGT41550.060.0595947
GTATGCC41750.059.85695645
GCCGTCT41700.059.59187749
CCGTCTT47550.052.55393650
TCTCGTA47200.052.5003741
ATCTCGT48600.050.98822440
TCACGAT51250.048.96497730
CACGATC51700.048.33599531
GCTTGAA54000.046.14588559
TGCTTGA54550.045.68071458
CTTGAAA65250.038.29681460
TCTGAAC73450.034.4508918
CGTCTGA75350.033.62884516
ACGTCTG76950.032.97488815
ACACGTC81850.031.00127813
CACACGT85750.029.67312212
GCACACG89250.028.4309811
Produced by FastQC (version 0.11.2)