FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20908620
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23750.058.65167646
CGTATGC24100.057.3655944
GTATGCC25650.057.03692645
GCCGTCT19700.055.96462649
ATGCCGT23050.055.4228947
TGCCGTC21750.055.35661348
GTCACAC50000.054.80963529
CACACTT54150.050.8031131
TCACACT54950.050.12681230
AATCGCG10950.049.22400739
ATCTCGT31800.048.09739340
TCTCGTA30300.047.82134641
AATCTCG33650.047.42967239
CCGTCTT26800.046.88356450
TCTGAAC69500.039.68261718
GAATCGC14000.039.0001138
ACGTCTG70600.038.866515
CGTCTGA71300.038.73036216
GCTTGAA34800.038.01580459
GCGTATG7200.036.9448243
Produced by FastQC (version 0.11.2)