FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21163480
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27900.058.5839446
CGTATGC28750.058.31426644
GTATGCC29100.058.21373745
TGCCGTC25950.057.05241848
ATGCCGT27500.056.76389347
GCCGTCT25750.055.3198549
CATCTCG33900.050.69428339
AGTCACC45300.049.68013828
ATCTCGT36200.046.215940
TCTCGTA35500.045.74646841
CCGTCTT32850.045.1739650
CACCAGA50400.044.9995631
GATCATC49350.044.18480736
ACCAGAT52800.042.7555732
CCAGATC57950.038.95663533
TGCTTGA39250.038.698858
GCTTGAA40850.038.5537759
TCATCTC46700.037.4735838
ACGTCTG63650.035.90678815
TCTGAAC64800.035.48526818
Produced by FastQC (version 0.11.2)