FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20006105
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1642240.8208694296066126TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC234900.11741415932786517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG187300.065.6266946
GTATGCC187800.065.52733645
CGTATGC188400.065.41186544
GCCGTCT185750.065.32641649
ATGCCGT189200.064.8011747
TGCCGTC189450.064.4761348
CCGTCTT196000.062.06989350
TCTCGTA199400.061.64495541
ATCTCGT202750.060.78176540
CGCCAAT205750.060.5085233
CACGCCA206400.060.43545531
GCCAATA208650.059.75139634
TCACGCC209550.059.46002630
CCAATAT222950.055.9657635
TGCTTGA217300.055.71108658
CAATATC222850.055.6609136
GCTTGAA220100.055.39975459
AATATCT237550.052.23125537
CTTGAAA243350.050.35114760
CTGCTTG248850.048.50721757
Produced by FastQC (version 0.11.2)