FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18517321
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1065850.5755962215052599TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC365570.19742056639834674TruSeq Adapter, Index 5 (98% over 50bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC234020.12637897242263066No Hit
CCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAA186460.10069491153714945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG164700.065.70486546
CGTATGC165500.065.6216544
GTATGCC167350.065.4814645
GCCGTCT156800.064.77573449
ATGCCGT164950.064.67274547
TGCCGTC161250.064.5512448
CCGTCTT168750.061.391150
TCTCGTA130600.061.02154541
CACAGTG199350.060.55446633
CAGTGAT198400.060.36843535
ATCTCGT133200.059.83075340
ACACAGT203950.059.29085532
AGTGATC145000.058.48652336
GTCACAC207550.058.27977429
TGCTTGA178000.057.4928358
GCTTGAA184000.056.3789159
TGTCGTA50550.056.22095541
ATGTCGT54800.052.62745340
CTTGAAA200150.052.40649860
TCTGAAC256950.047.78271518
Produced by FastQC (version 0.11.2)