FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18892287
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1781800.9431362121483757TruSeq Adapter, Index 4 (100% over 50bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC240380.1272371100439031No Hit
CCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAA193950.10266094306104921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG209550.064.4367646
CGTATGC210800.064.25526444
GCCGTCT208650.064.1772549
GTATGCC211250.064.0017345
ATGCCGT212000.063.7089447
TGCCGTC212600.063.21634748
TCTCGTA207000.062.27266341
CCGTCTT217300.061.7348250
ATCTCGT212600.060.58297740
AATCTCG213900.060.2813439
ACTGACC226750.060.1214832
TGCTTGA228650.058.45559358
GCTTGAA230850.058.18656559
AGTCACT238100.057.4023928
ACCAATC227350.056.82287236
CTTGAAA248800.054.22761560
TCTGAAC277800.049.5753618
CTGCTTG269900.049.50873657
CGTCTGA279900.049.1414216
TCGTATG281750.048.8199143
Produced by FastQC (version 0.11.2)