FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20478365
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC622640.3040477108401965TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG75950.062.81051646
GCCGTCT71050.062.31442349
CGTATGC77750.062.3024744
ATGCCGT75350.062.19648747
TGCCGTC73250.062.0208548
GTATGCC79400.061.80122445
CCGTCTT80800.056.5707450
GCATCTC87950.056.50861738
TCTCGTA85300.056.4590741
CATCTCG86800.056.45181339
TAGGCAT101350.055.8068135
ATCTCGT89650.054.30542440
CACTTAG107950.052.6871331
GCTTGAA92750.049.2431759
TGCTTGA91700.049.19636558
AGTCACT116600.048.86845828
TCACTTA121400.047.10936430
CTTGAAA110600.042.11844660
TCTGAAC147450.039.02182418
ACGTCTG148600.038.72030615
Produced by FastQC (version 0.11.2)