FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19671758
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC633170.3218675219571123TruSeq Adapter, Index 2 (100% over 50bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC220370.11202354156654429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82150.063.0553644
GTATGCC84400.062.86694745
ATGCCGT79100.062.43328547
TATGCCG82500.062.4052446
GCCGTCT73650.062.30076649
TGCCGTC75700.062.00113748
CCGATGT114300.058.822833
TCTCGTA91650.057.97051241
ACCGATG117150.057.48139632
ATCTCGT98450.055.42433540
CCGTCTT86300.055.39861750
GATGTAT122300.055.23287235
GTCACCG123150.054.8509529
TCACCGA124100.054.40271430
AGTCACC124500.054.1999528
TGCTTGA97050.049.83836758
GCTTGAA99500.049.52535259
TCTGAAC161100.042.3636818
CGTCTGA161150.042.19925716
ACGTCTG161400.042.0469415
Produced by FastQC (version 0.11.2)