FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_sl19chipbzip1_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_sl19chipbzip1_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21487661
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC393050.1829189319395908TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52200.059.4051946
GCCGTCT52250.059.2150649
CGTATGC52400.059.18092744
ATGCCGT52850.058.6090247
GTATGCC53050.058.3887445
TGCCGTC53600.057.854548
TCTCGTA56450.053.19828441
CCGTCTT60350.051.6723950
CACGATC59500.050.2948436
ATCTCGT59800.050.2185840
TCACGAT62100.049.4854135
ATCACGA65800.046.8096234
TGCTTGA72150.042.83289758
GCTTGAA72550.042.7411659
GTCACAT73250.042.5259129
CTTGAAA90450.034.4373360
TCTGAAC103750.030.25947818
CGTCTGA105200.029.7424616
ACGTCTG107000.029.27462215
ACACGTC118050.026.56409613
Produced by FastQC (version 0.11.2)