FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36683496
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG1173400.3198713666767202No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA856020.2333528952638538No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG572300.15601021233090762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT459500.018.066768
ACGAGTT526450.015.80897869
GGGGCCC14200.014.2951570
CCACGAG602350.013.93313167
GCCACGA607850.013.85889966
GGCCACG612150.013.64735165
GCGAGGA363400.012.7028831
GGATAAC697450.012.3686741
ACGGCCA681750.012.35146263
CGGCCAC690900.012.21329564
AACGGCC687650.012.16917662
GAACGGC708550.011.79540561
GTGAACG717750.011.62478959
TGAACGG721100.011.61930360
GCGGGGC22650.011.5975131
ACATGGC742150.011.5598816
CGTGAAC730000.011.45360958
GGCCATC743550.011.30124610
TAACATG767350.011.1483064
CATGGCC764650.011.1265687
Produced by FastQC (version 0.11.2)