FastQCFastQC Report
Wed 27 Apr 2016
H5YHGBGXY_n01_crf4roots_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5YHGBGXY_n01_crf4roots_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16805061
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG402850.2397194511819981No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA293910.17489374183170175No Hit
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG186940.11124029838392137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT178950.016.8194768
ACGAGTT210750.014.34784169
GGCCACG234900.012.94766265
CCACGAG236700.012.90811467
GCGGGGA31100.012.8379721
GCCACGA237750.012.82166766
GCGAGGA138950.012.3002291
GTATGCC17850.012.15702947
AACGGCC257200.011.85224262
GGATAAC265100.011.8504441
ACGGCCA258250.011.83115863
CGGCCAC265500.011.48175464
TGAACGG265350.011.44850460
GAACGGC264550.011.43020761
ACATGGC272850.011.3277346
GGGGCCC6800.011.32280870
GTGAACG268800.011.30156559
GGCCATC268250.011.27241510
CGCGGGG11700.011.0756141
ATGCGCT271850.011.02073829
Produced by FastQC (version 0.11.2)